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. 2023 Nov 20;123(1):68–79. doi: 10.1016/j.bpj.2023.11.019

Figure 5.

Figure 5

CD spectra for DsbB reconstituted in a dark nanodisc (DMPC/DMPG) and empty dark nanodiscs (DMPC/DMPG) in the absence of DsbB at different temperature points along a thermal gradient. CD spectra were collected over a thermal ramp ranging from 25°C to 95°C at a step of 1°C/min in the 190- to 280-nm UV region. The CD spectra for each sample are shown at 95°C (red), 65°C (orange), and 25°C (blue). DsbB reconstituted in a dark nanodisc is shown as a solid line and the empty nanodisc is shown as a dotted line at each respective temperature. All CD scans were done in triplicate and the average is plotted.