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. 2023 Dec 6;123(2):157–171. doi: 10.1016/j.bpj.2023.12.004

Figure 2.

Figure 2

ROCK activity is sufficient for myosin clusters to grow in the absence of myosin motor activity. (A) Top row shows representative myosin images of cells with or without ROCK activity and myosin motor activity. In the left panel, myosin clusters were disassembled by both Y-27632 and blebbistatin (Y+B+). The middle panel shows cases where Y27 or blebbistatin are removed individually (Y+B– and Y–B+). The right panel shows the control condition where both inhibitors are removed (Y–B–). Zoomed in myosin images are shown in the bottom row. Images are inverted. Scale bars, 10 μm (top row) and 2 μm (bottom row). (B) Histogram of myosin cluster size between Y27/blebbistatin-treated and blebbistatin-treated cells. Inset shows the cumulative distribution of cluster sizes. (C) The sum of myosin cluster sizes. (D) Mode of myosin cluster size distribution. (E) Density of myosin clusters. n = 20 cells for each condition. Two-sample Kolmogorov-Smirnov tests were performed in (C–E) to test if the measured quantity differs between conditions. n.s. signifies p > 0.05, ∗ signifies p < 0.05, ∗∗ signifies p < 0.01, ∗∗∗ signifies p < 0.001. To see this figure in color, go online.