Figure 4.

Myosin motor activity allows for myosin cluster growth. (A) Top row shows representative myosin images in cells treated with or without blebbistatin. Bottom row shows zoomed-in myosin images in the red boxes in the top row. Images are inverted. Scale bars, 10 μm (top row) and 2 μm (bottom row). (B) Histogram of myosin cluster sizes between blebbistatin-treated and control cells. Inset shows the cumulative distribution of both conditions. (C) The sum of all myosin cluster sizes. (D) Mode of myosin cluster size distribution. (E) Density of myosin clusters. n = 20 cells for blebbistatin-treated cells and Nn= 18 cells for control cells. Two-sample Kolmogorov-Smirnov tests were performed in (C–E) to test if the measured quantity differs between conditions. n.s. signifies p > 0.05, ^∗∗ signifies p < 0.01, ^∗∗∗ signifies p < 0.001. To see this figure in color, go online.