Figure 6.
Myosin cluster sizes correlate with F-actin bundle intensity under myosin motor activity. (A) F-Actin and myosin on select F-actin bundles in control cells. Left column shows all segments of F-actin bundles that are selected for analysis. Right column shows zoomed-in view of a few F-actin bundles. Solid lines represent F-actin bundles where actomyosin correlation is examined. Arrowheads indicate the end points of the segment. (B) F-Actin and myosin on select F-actin bundles in blebbistatin-treated cells. Scale bars, 10 μm (whole cell image) and 2 μm (zoomed-in image). (C) Correlation between myosin cluster size and F-actin bundle intensity in control and blebbistatin-treated cells. Correlation is plotted for myosin clusters on every selected bundle in a single cell. (D) Slope of the correlation. n = 5 cells in each condition. The two-sample t-test is performed for statistical analysis. ∗∗∗ signifies p < 0.001. (E) R2 of the correlation. n = 5 cells in each condition. The two-sample t-test is performed for statistical analysis. ∗∗∗ signifies p < 0.001. To see this figure in color, go online.