Figure 7.

Myosin association to F-actin bundles depends on F-actin architecture. (A) F-Actin and myosin on select F-actin bundles in control cells. (B) F-Actin and myosin on select F-actin bundles in LatA-treated cells. Scale bars 10 μm (whole cell image) and 2 μm (zoomed-in image). (C) Histogram of myosin cluster sizes between LatA-treated and control cells. Inset shows the cumulative distribution of both conditions. (D) The sum of all myosin cluster sizes. (E) Mode of myosin cluster size distribution. (F) Density of myosin clusters. n = 18 cells for LatA-treated cells and n = 18 cells for control cells. Two-sample Kolmogorov-Smirnov tests were performed in (D–F) to test if the measured quantity differs between conditions. n.s. signifies p > 0.05, ^∗∗ signifies p < 0.01, ^∗∗∗ signifies p < 0.001. (G) Correlation between myosin cluster intensity and F-actin bundle intensity in control and LatA-treated cells. Correlation is plotted for myosin clusters on every selected bundle in a single cell. Inset shows the R² of the correlation between control and LatA-treated cells. n = 5 cells in each condition. The two-sample t-test is performed for statistical analysis. n.s., p > 0.05. To see this figure in color, go online.