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. 2023 Dec 6;123(2):157–171. doi: 10.1016/j.bpj.2023.12.004

Figure 8.

Figure 8

Myosin cluster growth with positive feedback is sufficient to recapitulate the broad range of myosin cluster sizes. (A) Schematic of the simulation setup. (B) Simulation of myosin cluster growth without myosin motor activity. (i) Experimentally observed myosin cluster size distribution in cells before (Y+B+) and after (Y–B+) washing out Y-27 in cells that are treated with both Y-27 and blebbistatin. (ii) Simulation when myosin binding probability is uniform across all grid points (α = 0). Inset shows the cumulative distribution of the experimental and simulated results. (iii) Simulation when myosin has an affinity toward existing myosin clusters (α = 10). Inset shows the cumulative distribution of the experimental and simulated results. (iv) Average distance between cumulative distributions of the experimental and simulated cluster sizes across different α and Nmyo values. Stars show the Nmyo determined by experimental quantifications. (C) Simulation of myosin cluster growth with myosin motor activity. (i) Experimentally observed myosin cluster size distribution in cells treated with blebbistatin or control cells. (ii) Simulation when myosin binding probability is uniform across all grid points (α = 0). Inset shows the cumulative distribution of the experimental and simulated results. (iii) Simulation when myosin has an affinity toward existing myosin clusters (α = 10). Inset shows the cumulative distribution of the experimental and simulated results. (iv) Average distance between cumulative distributions of the experimental and simulated cluster sizes across different α and Nmyo values. Stars show the Nmyo values determined by experimental quantifications. To see this figure in color, go online.