Extended Data Fig. 3. ABA treatment increases suberin levels but not localization in Solanum lycopersicum, while levels remain unchanged in Solanum pennellii.

(a) Fluorol yellow staining of 7-day-old S. lycopersicum wild-type plants treated with mock or 1 µM ABA for 48 h. Cross-sections of roots at 1 cm from the hypocotyl junction. (b) Fluorol yellow staining for suberin in tomato wild relative S. pennellii (LA0716) 7-day-old plants treated with mock or 1 µM ABA for 48 h. Whole-mount staining of primary roots. (c) Developmental stages of suberin deposition of plants treated with mock or 1 µM ABA for 48 h. Zones were classified in non-suberized (white), patchy suberized (gray) and continuously suberized (yellow), n = 7, error bars: SD.