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. 2024 Jan 2;10(1):118–130. doi: 10.1038/s41477-023-01567-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — (a) Graphical depiction of a tomato root section with cell types profiled in the single cell population. (b) A pseudo-time trajectory analysis ran on the population. (c) Annotation of single cell clusters displayed by an integrated uniform manifold approximation and projection (UMAP). Circles indicate subpopulations clustered together. (d) Reproducibility of biological replicates as observed by UMAP and cluster identification. (e) Expression profiles for 39 genes expressed across the major root tissue types. Dot diameter represents the percentage of cells in which each gene is expressed (% Exp.); and colors indicate the average scaled expression of each gene in each developmental stage group with warmer colors indicating higher expression levels. Top row indicates whether the gene’s expression has been validated in S. lycopersicum in previously published work. R.C.: Root cap. Q.C.: Quiescent center. Col: Columella. Procamb: Procambium. Phlo: Phloem.