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. 1991 Jun;96(2):584–587. doi: 10.1104/pp.96.2.584

Effect of Gabaculine on the Synthesis of Heme and Cytochrome f in Etiolated Wheat Seedlings 1

Clare M Anderson 1, John C Gray 1
PMCID: PMC1080810  PMID: 16668225

Abstract

The effect of gabaculine (3-amino 2,3-dihydrobenzoic acid), an inhibitor of tetrapyrrole synthesis, on the accumulation of heme and cytochrome f in etiolated wheat (Triticum aestivum var Mardler) seedlings has been examined. Gabaculine treatment resulted in decreased amounts of heme and of holocytochrome f detected spectroscopically and by peroxidase activity after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The amount of the cytochrome f polypeptide detected immunochemically on Western blots was much less affected by gabaculine treatment, indicating that apocytochrome f synthesis was not tightly coupled to heme availability. Gabaculine treatment did not affect the size of the cytochrome f polypeptide, indicating that heme addition is not required for proteolytic removal of the presequence.

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Selected References

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  1. Bennett J. Biosynthesis of the light-harvesting chlorophyll a/b protein. Polypeptide turnover in darkness. Eur J Biochem. 1981 Aug;118(1):61–70. doi: 10.1111/j.1432-1033.1981.tb05486.x. [DOI] [PubMed] [Google Scholar]
  2. Castelfranco P. A., Jones O. T. Protoheme turnover and chlorophyll synthesis in greening barley tissue. Plant Physiol. 1975 Mar;55(3):485–490. doi: 10.1104/pp.55.3.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Doherty A., Gray J. C. Synthesis of cytochrome f by isolated pea chloroplasts. Eur J Biochem. 1979 Jul;98(1):87–92. doi: 10.1111/j.1432-1033.1979.tb13164.x. [DOI] [PubMed] [Google Scholar]
  4. Elich T. D., Lagarias J. C. Phytochrome Chromophore Biosynthesis : Both 5-Aminolevulinic Acid and Biliverdin Overcome Inhibition by Gabaculine in Etiolated Avena sativa L. Seedlings. Plant Physiol. 1987 Jun;84(2):304–310. doi: 10.1104/pp.84.2.304. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gasser S. M., Ohashi A., Daum G., Böhni P. C., Gibson J., Reid G. A., Yonetani T., Schatz G. Imported mitochondrial proteins cytochrome b2 and cytochrome c1 are processed in two steps. Proc Natl Acad Sci U S A. 1982 Jan;79(2):267–271. doi: 10.1073/pnas.79.2.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gray J. C. Purification and properties of monomeric cytochrome f from charlock, Sinapis arvensis L. Eur J Biochem. 1978 Jan 2;82(1):133–141. doi: 10.1111/j.1432-1033.1978.tb12004.x. [DOI] [PubMed] [Google Scholar]
  7. Hauska G., Hurt E., Gabellini N., Lockau W. Comparative aspects of quinol-cytochrome c/plastocyanin oxidoreductases. Biochim Biophys Acta. 1983 Jul 15;726(2):97–133. doi: 10.1016/0304-4173(83)90002-2. [DOI] [PubMed] [Google Scholar]
  8. Jones A. M., Allen C. D., Gardner G., Quail P. H. Synthesis of phytochrome apoprotein and chromophore are not coupled obligatorily. Plant Physiol. 1986 Aug;81(4):1014–1016. doi: 10.1104/pp.81.4.1014. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Klein R. R., Gamble P. E., Mullet J. E. Light-Dependent Accumulation of Radiolabeled Plastid-Encoded Chlorophyll a-Apoproteins Requires Chlorophyll a: I. Analysis of Chlorophyll-Deficient Mutants and Phytochrome Involvement. Plant Physiol. 1988 Dec;88(4):1246–1256. doi: 10.1104/pp.88.4.1246. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Klein R. R., Mullet J. E. Control of gene expression during higher plant chloroplast biogenesis. Protein synthesis and transcript levels of psbA, psaA-psaB, and rbcL in dark-grown and illuminated barley seedlings. J Biol Chem. 1987 Mar 25;262(9):4341–4348. [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. May T. B., Guikema J. A., Henry R. L., Schuler M. K., Wong P. P. Gabaculine Inhibition of Chlorophyll Biosynthesis and Nodulation in Phaseolus lunatus L. Plant Physiol. 1987 Aug;84(4):1309–1313. doi: 10.1104/pp.84.4.1309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nicholson D. W., Stuart R. A., Neupert W. Biogenesis of cytochrome c1. Role of cytochrome c1 heme lyase and of the two proteolytic processing steps during import into mitochondria. J Biol Chem. 1989 Jun 15;264(17):10156–10168. [PubMed] [Google Scholar]
  14. Ohashi A., Gibson J., Gregor I., Schatz G. Import of proteins into mitochondria. The precursor of cytochrome c1 is processed in two steps, one of them heme-dependent. J Biol Chem. 1982 Nov 10;257(21):13042–13047. [PubMed] [Google Scholar]
  15. Plesnicar M., Bendall D. S. The photochemical activities and electron carriers of developing barley leaves. Biochem J. 1973 Nov;136(3):803–812. doi: 10.1042/bj1360803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rothstein S. J., Gatenby A. A., Willey D. L., Gray J. C. Binding of pea cytochrome f to the inner membrane of Escherichia coli requires the bacterial secA gene product. Proc Natl Acad Sci U S A. 1985 Dec;82(23):7955–7959. doi: 10.1073/pnas.82.23.7955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Takabe T., Takabe T., Akazawa T. Biosynthesis of P700-Chlorophyll a Protein Complex, Plastocyanin, and Cytochrome b(6)/f Complex. Plant Physiol. 1986 May;81(1):60–66. doi: 10.1104/pp.81.1.60. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Thomas P. E., Ryan D., Levin W. An improved staining procedure for the detection of the peroxidase activity of cytochrome P-450 on sodium dodecyl sulfate polyacrylamide gels. Anal Biochem. 1976 Sep;75(1):168–176. doi: 10.1016/0003-2697(76)90067-1. [DOI] [PubMed] [Google Scholar]
  19. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Werck-Reichhart D., Jones O. T., Durst F. Haem synthesis during cytochrome P-450 induction in higher plants. 5-Aminolaevulinic acid synthesis through a five-carbon pathway in Helianthus tuberosus tuber tissues aged in the dark. Biochem J. 1988 Jan 15;249(2):473–480. doi: 10.1042/bj2490473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Willey D. L., Auffret A. D., Gray J. C. Structure and topology of cytochrome f in pea chloroplast membranes. Cell. 1984 Feb;36(2):555–562. doi: 10.1016/0092-8674(84)90248-4. [DOI] [PubMed] [Google Scholar]

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