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Dose-response profile of PspA–GM-CSF injected i.n.a
| Immunogen | Amt (U) of cyto- kine injected | Amt (μg) of protein) | Mean IgG1 anti-PspA titer (SE) |
|---|---|---|---|
| PspA | 37 | 246 | |
| PspA–GM-CSF | 80,000 | 37 | 65,476 (1.40) |
| 40,000 | 18 | 29,060 (2.71) | |
| 20,000 | 9 | 12,823 (1.65) | |
| 10,000 | 4.5 | 143 (2.79) |
Groups of five DBA/2 mice were immunized i.n. with PspA (37 μg) or different doses of PspA–GM-CSF on days 0, 7, and 14. Mice were bled 14 days later, and anti-PspA titers were determined by ELISA.
