TABLE 5.
Comparison of i.n. immunization with PspA-cytokine fusions in anesthetized and nonanesthetized micea
| Immunogen | Mean IgG1 anti-PspA titer (SE)
|
|
|---|---|---|
| Anesthetizeda | Nonanesthetizedb | |
| PspA | <1,000 | <1,000 |
| PspA–IL-2 | 31,655 (1.44) | 16,656 (1.51) |
| PspA–GM-CSF | 22,730 (1.75) | 9,363 (1.49) |
a
Groups of five DBA/2 mice were immunized i.n. with PspA–IL-2 (5 μg), PspA–GM-CSF (5 μg), or PspA (5 μg) on days 0, 7, and 14. Mice were bled 14, 28, and 44 days later, and anti-PspA titers were determined by ELISA.
b
Mice were anesthetized with CO2.