TABLE 6.
Anti-PspA responses in mice stimulated by s.c. injection of different doses of PspA–IL-2 or PspA–GM-CSFa
| Immunogen | Dose (μg/mouse) | IgG1 anti-PspA antibody titer
|
|
|---|---|---|---|
| Day 14 | Postboost day 14 | ||
| PspA | 5 | <50 | <50 |
| 0.05 | <5 | <5 | |
| PspA–IL-2 prepn 1 | 5 | 559 | >40,000 |
| 0.5 | 188 | 11,639 | |
| 0.05 | 15 | 183 | |
| PspA–GM-CSF | 5 | 3,883 | 35,852 |
| 0.5 | <10 | 6,032 | |
| 0.05 | <10 | 137 | |
a
Groups of five DBA/2 mice were immunized with PspA–IL-2 or PspA–GM-CSF at 5, 0.5, or 0.05 μg/mouse. Mice were boosted on day 14 and bled 14 days later. Results represent arithmetic means of ELISA titers determined for individual sera. Results are from one of four representative experiments.