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. 2024 Jan 24;14:2124. doi: 10.1038/s41598-024-52598-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of slit-lamp photography and AS-OCTA imaging for corneal vascularization. Examples of eyes (respective image rows) with corneal vascularization imaged with slit-lamp photography (panels A: visualized corneal area delineated with Fiji, see Methods, region of interest imaged by AS-OCTA highlighted with a red rectangle); panels B: segmentation of corneal vascularization using the morphological hessian-based method; panels C: segmentation of corneal vascularization using Gabor filtering), and 3 × 3-mm AS-OCTA (panels D: AS-OCTA en-face scan after post-processing in Fiji: capillary flow area delineated in white; panels E: AS-OCTA cross-sectional scan showing a vessel flow signal in red with its marked (blue) deepest extension in relation to total corneal thickness).