Fig. 5.

Overexpression of TFEB/TFE3 attenuates lysosomal dysfunction in arsenic-exposed primary BMDCs. Primary BMDCs were electro-transfected with plasmid carrying exogenous TFEB/TFE3 or empty vector and then exposed to 1 μM As for 12 h. A Immunofluorescence images stained with Lyso-Tracker Red or Lyso-Sensor Green and exposed to As with TFEB overexpression. B, C Fluorescence intensity of Lyso-Tracker Red or Lyso-Sensor Green quantification, respectively (n = 4). D Western blotting of lysosome-associated proteins exposed to As with TFEB overexpression. E Immunofluorescence images stained with Lyso-Tracker Red or Lyso-Sensor Green and exposed to As with TFE3 overexpression. F, G Fluorescence intensity of Lyso-Tracker Red or Lyso-Sensor Green quantification, respectively (n = 4). H Western blotting of lysosome-associated proteins exposed to As with TFE3 overexpression. All summary data are normalized to negative control (NC), and values reflect Mean ± SEM. Scale bar: 20 μm. ^*P < 0.05 compared with NC group, ^#P < 0.05 compared with NC + As group