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. 2024 Jan 24;15:683. doi: 10.1038/s41467-024-44789-z

Fig. 2. Differential expression of CD11b and CD49a capture temporal changes in NK cell phenotype.

Fig. 2

A Expression of CD49a vs CD49b by NK cells (CD3- NK1.1+ ) isolated from MC38 tumors grafted subcutaneously on the flank. B Expression of CD49a vs CD11b by NK cells isolated from MC38 tumors, alongside blood. C Expression of CD49a vs CD11b by NK cells across multiple tumor models. NK cells identified as CD3- NK1.1+ in C57BL/6 mice, CD3- NKp46+ in BALB/c mice. D Proportion of NK cells within the CD11b+ CD49a-, CD11b- CD49a- (double negative, DN) and CD11b- CD49a+ gates across the different tumor models. Data collected from B16-F10-OVA and EO771 tumors was from 1 independent repeat, and MC38, CT26, and MMTV-PyMT tumors pooled from 2 independent repeats, where MC38 (24 h n = 12, 72 h n = 12), CT26 (24 h n = 8, 72 h n = 8), EO771 (24 h n = 4, 72 h n = 4), B16-F10-OVA (24 h n = 4, 72 h n = 4), and MMTV-PyMT (24 h n = 7, 72 h n = 12). E Number of Kaede Green+ and Kaede Red+ NK cells at 5, 24 and 72 h post photoconversion of MC38 tumors. F Expression of CD49a versus CD11b by Kaede Green+ and Kaede Red+ NK cells at 5, 24 and 72 h post photoconversion. G The proportion of Kaede Green/Red for each NK cell subset at each time point post photoconversion. Data at 5 (n = 5) h is representative of 1 independent repeat, whereas 24 (n = 8) and 72 (n = 11) h pooled from 2 independent repeats. H UMAPs showing protein expression of CD11b and CD49a alongside Kaede Green/Red expression by NK cells isolated from MC38 tumors at 24 and 72 h post photoconversion. I UMAPS showing expression of CD11b, CD49a, NKG2A, LAG-3 and CD69. J Enumeration of the proportion of cells expressing NKG2A, LAG-3 and CD69 across the NK cell subsets. Data in UMAPs 24 h (n = 5), and 72 h (n = 8) are representative of two independent repeats. Statistical significance was determined by two-way ANOVA with Šidák’s multiple comparisons test (J). Data are presented showing all individual data points as well as the mean value +/- SD. In all experiments, ‘n’ defines a single tumor on an individual mouse, i.e., n = 3 refers to 3 mice each with a single tumor.