FIG. 3.

Inhibition of iNOS mRNA expression by salivary gland lysates of P. papatasi. Mφ were treated for 8 h with IFN-γ (25 U/ml) and LPS (25 ng/ml) in the presence or absence of saliva. Total RNA was extracted, subjected to RT-PCR, electrophoresed, and analyzed by optical densitometry. Lanes 1 to 4, HPRT; lanes 5 to 8, iNOS. Lanes marked +S include saliva. Results are from one representative experiment of three. M, size markers.