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. 1998 Apr;66(4):1534–1537. doi: 10.1128/iai.66.4.1534-1537.1998

FIG. 4.

FIG. 4

Inhibition of PP activity (A) and NO production (B) by whole P. papatasi saliva and by different size fractions obtained by filtration through molecular size cutoff filters. S, whole saliva; <3, <3-kDa filtrate; 100, 100-kDa retentate. (A) PP-1 and PP-2A inhibition assays were performed with 250 ng of untreated Mφ cytosolic fraction and two gland equivalents per assay. (B) For NO analysis, Mφ (106 cells/ml/well) were activated by using LPS in the presence of different fractions for 24 h. NO2 was assayed as described in Materials and Methods. Data shown are means ± standard deviations of triplicate experiments.