FIG. 7.

T-cell Western analysis of splenocytes from Neospora-vaccinated mice. Toxoplasma (A) and Neospora (B) lysates were separated by Western blotting as described in Materials and Methods. Splenocytes were isolated from Neospora-vaccinated mice, and the CD8⁺ population (95% pure) was incubated in the presence of the parasite antigen plus irradiated feeder cells. Lymphoproliferation in response to the parasite extract was determined by measuring thymidine incorporation. Molecular weights are in thousands. Error bars indicate SD.