TABLE 1.
Phenotypic analysis of splenocytes postvaccination and postchallengea
| Treatment | Absolute no. of cells of indicated cell type (107) ± SD
|
|||
|---|---|---|---|---|
| CD4 | CD8 | γδ | NK | |
| Saline | 1.5 ± 0.04 | 0.85 ± 0.03 | 0.16 ± 0.02 | 0.17 ± 0.04 |
| Neospora | 1.62 ± 0.08 | 1.49 ± 0.09 | 0.15 ± 0.04 | 0.65 ± 0.05 |
| Toxoplasma | 0.92 ± 0.03 | 1.12 ± 0.17 | 1.32 ± 0.04 | 0.75 ± 0.14 |
| Neospora + Toxoplasma | 1.64 ± 0.05 | 2.0 ± 0.33 | 1.25 ± 0.06 | 0.96 ± 0.11 |
a
Mice were infected with 106 N. caninum tachyzoites and challenged with 104 PLK strain tachyzoites of T. gondii. Phenotypic expression of CD4, CD8, γδ, and NK cells was determined either at the time of challenge (14 days postinfection with Neospora) or 7 days after Toxoplasma challenge. n = 4 mice/group.