TABLE 2.
Analysis of B. bigemina RAP-1 peptide sequences for amphipathicity
| Peptide | Sequence | Amphipathic scorea | Able to stimulate T-cell clones? |
|---|---|---|---|
| RAP-1 CT-1 aa 456–480 | DAENEVRADDPKNEDSSSSSDDSDA | 5.0, 4.4 | No |
| AAA........AA............ | |||
| RAP-1 CT-1 aa 436–465 | VNSEKVDADDAGNAETQQLPDAENEVRADD | 20.0, 5.0 | Yes |
| .......AAAAAAAAAAAA.AAA....... | |||
| RAP-1 aa 159–187 | FVVSLLKKNVVRDPESNDVENFASQYFYM | 5.3, 5.2, 28.4 | Yes |
| AAA.AAAA.AAAAAAAAAAAAAAA..... |
a
The whole RAP-1α1 protein was analyzed with the AMPHI algorithm (13, 22) for alpha-helical periodicity and amphipathicity and assigned an amphipathic score for each set of amino acids that scored. Analysis of the peptides used in T-cell proliferation assay is presented, and the region of sequence with predicted antigenic T-cell sites is indicated below the amino acid sequence by the letter A. The oligopeptide motifs conserved in P. falciparum AMA-1 and B. bigemina RAP-1 are in boldface.