Fig. 2.
Ecm1 deletion in hepatocytes weakened the therapeutic effects of Sal B on liver fibrosis. (A) Scheme of the experimental procedure for WT and Ecm1Δhep mice injected with CCl4 and treated with Sal B, Sora or Cile. (B) Serum ALT and AST activity. (C) Representative images of H&E and SR staining (bar = 100 μm), and semiquantitative analysis of the SR-positive staining area. (D) Hyp levels in liver tissue. (E) Western blot analysis and quantification of α-SMA protein expression using gray value analysis, Acta2 and Col1a1 mRNA expressions, and IHC staining of α-SMA and Col-I (bar = 100 μm). (F) TGF-β1 activity in liver tissue. Data are represented as mean ± SD (n = 4–6/group). *P < 0.05; **P < 0.01; ***P < 0.001.