Fig. 4.

Ecm1 interacted with xCT to affect hepatocyte ferroptosis. (A) Representative images of H&E, Masson and SR staining (bar = 50 μm) and TEM images (bar = 0.5 μm) of WT and Ecm1-KO mice. (B) Western blot analysis and quantification of xCT protein expression in WT and Ecm1-KO mice using gray value analysis. (C) Representative images of immunofluorescence co-staining of Ecm1 and xCT (bar = 50 μm). (D) Ecm1 interacts with xCT, as shown by co-IP. Data are represented as mean ± SD (n = 3/group). ***P < 0.001.