Fig. 5.
Deleting Ecm1 in hepatocytes reduced Sal B-mediated upregulation of xCT transporters and inhibition of iron-dependent lipid peroxidative injury. In WT and Ecm1Δhep mice, (A) Fth1, Ftl, Acsl4 and Ptgs2 mRNA expressions. (B) Hepatic iron, 4-HNE, MDA and GSH levels. (C) Representative images of IHC staining of Fth1, Tfrc, and Gpx4 (bar = 100 μm). (D) Western blot analysis and quantification of Tfrc, xCT, and Gpx4 protein expression using gray value analysis. Data are represented as mean ± SD (n = 4–6/group). *P < 0.05; **P < 0.01; ***P < 0.001.