Fig. 6.

Both Sal B and ECM1 treatment inhibited hepatocyte ferroptosis. (A) Scheme of the experimental procedure for LO2 cells stimulated with erastin and treated with Sal B or ECM1. (B) ROS intensity in hepatocytes. (C) ROS staining in hepatocytes (bar = 50 μm). (D) Ptgs2 and Acsl4 mRNA expression. (E) and (F) Western blot analysis and quantification of Ecm1, xCT, and Gpx4 protein expression using gray value analysis. Data are represented as mean ± SD (n = 3/group). *P < 0.05; **P < 0.01; ***P < 0.001.