Fig. 7.

Ecm1 deletion abrogated the inhibitory effect of SalB on hepatocyte ferroptosis. (A) Scheme of the experimental procedure for Sh-NC and Sh-Ecm1 LO2 cells stimulated with erastin and treated with Sal B or ECM1. (B) ROS activity (bar = 50 μm). (C) MDA and GSH levels. (D) Acsl4 and Ptgs2 mRNA expression. (E) and (F) Western blot analysis and quantification of Ecm1, xCT, and Gpx4 protein expression using gray value analysis. Data are represented as mean ± SD (n = 3/group). *P < 0.05; **P < 0.01; ***P < 0.001.