Fig. 4.

PHAR prevents the development of NASH and fibrosis. A, histochemical analysis of H&E, Oil red O (neutral fat), Sirius red. (collagen I and III), and immunohistochemistry for F4/80. Black arrow in H&E photographs points an area with an inflammatory focus; orange arrow indicates an area of ballooning. B–C, steatosis, inflammation, and ballooning, and integration of the three parameters in the NAFLD activity score (NAS). Data are mean ± S.D. (n = 6–7). *p < 0.01; **p < 0.01 vs. STAM vehicle according to a two-way ANOVA followed by Bonferroni post-hoc test. D, quantification of Oil red O positive area. Data are mean ± S.D. (n = 6–7). **p < 0.01 vs STAM vehicle according to a two-way ANOVA test. E, quantification of Sirius red positive area. Data are mean ± S.D. (n = 6–7). *p < 0.05 vs. STAM-VEH according to a two-way ANOVA followed by Bonferroni post-hoc test. F, Quantification of DAB-staining positive area of F4/80. Data are mean ± S.D. (n = 6–7). ***p < 0.001 vs. CTRL-VEH or STAM-VEH according to a two-way ANOVA test. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)