Fig. 5.

PHAR counteracts the fibrotic transcriptional signature. A, Changes in expression of 28 out of 84 genes involved in liver fibrosis were analyzed through RT² Profiler™ PCR Array Mouse Fibrosis Qiagen for CTRL-VEH, CTRL-PHAR, STAM-VEH and STAM-PHAR livers. The 28 genes showed significant expression changes between CTRL-VEH vs. STAM-VEH (n = 4 per experimental group; p-value ≤0.05; two-way ANOVA, Bonferroni's post-hoc test). These genes were grouped by their biological function in a heatmap, representing the individual log₂ of the fold of change. The color scale is capped at −2 and 2 of log₂ fold of change. B, Volcano plot representing -log₁₀ of p-value (calculated by a two way-ANOVA, Bonferroni's post-hoc test) and log₂ of the fold of change for differences in gene expression between STAM-VEH and STAM-PHAR. The threshold p-value (dashed horizontal line) was established to <0.05, and the threshold for log₂-fold change (dashed vertical lines) was established at -log₂(1.5) and log₂(1.5). Genes surpassing both thresholds are shown in red. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)