FIGURE 2.

Cyclic stretch loading suppresses YAP phosphorylation and downregulates the key Hippo pathway, LATS phosphorylation. (A,B) MSCs were stretched and YAP phosphorylation was assessed by western blotting. The expression levels of p-YAP (S127) and p-YAP (S397) were decreased by stretch, indicating that YAP cytoplasmic retention via phosphorylation was suppressed by stretch. The ratio of p-YAP/total YAP was quantified (n = 4). (C,D) The phosphorylation of LATS is a key Hippo pathway that acts as a potential anti-YAP upstream effector. LATS phosphorylation was impaired under cyclic stretch loading for 30 min. The ratio of p-LATS1/total LATS1 was quantified (n = 3). NS: non-significant, *: p < 0.05, **: p < 0.01, and ***: p < 0.001 compared with control.