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. 2024 Jan 11;11:1306002. doi: 10.3389/fbioe.2023.1306002

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Copyright © 2024 Kim, Riehl, Bouzid, Yang, Duan, Donahue and Lim.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Stretch loading induction of MSC osteogenesis is suppressed by ROCK inhibitor. MSCs were stretched without or with ROCK inhibitor (Y27632). (A) Osteogenic gene expressions by qRT-PCR on day 7 (n = 4). (B,C) Alizarin red bone mineral staining on day 21 (n = 3). (D,E) Western blotting of RUNX2 expression on day 7 (n = 3). NS: non-significant; *: p < 0.05, **: p < 0.01, and ***: p < 0.001 compared with control or corresponding counterpart; ^##: p < 0.01 for comparison between stretch vs. stretch + YAP siRNA.