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. Author manuscript; available in PMC: 2024 Jan 25.
Published in final edited form as: Mucosal Immunol. 2023 Sep 15;16(6):826–842. doi: 10.1016/j.mucimm.2023.09.003

Fig. 8.

Fig. 8

TQ reduces signaling along the IL-6-IL-6R axis in T cells. (A) In the human T cell line, Jurkat cells, TQ reduced the IL-6Rα/CD126 protein levels. The GAPDH levels are shown as the loading control. Representation of 3 blots. (B) Densitometric quantification of IL-6Rα/CD126 protein bands normalized to GAPDH from (A). (C) TQ, but not other AhR agonists FICZ and β-NF, reduced the levels of IL-6Rα/CD126 in Jurkat cells. The GAPDH levels are shown as the loading control. Representation of 3 blots. (D) Densitometric quantification of IL-6Rα/CD126 protein bands normalized to GAPDH from (C). (E) TQ reduced the IL-6-induced upregulation of luciferase activity in Jurkat cells where the luciferase reporter was placed downstream of the Stat-3 dimer binding site. The AhR agonists FICZ and β-NF did not affect the IL-6-induced luciferase activity. Representation of 2 independent experiments with 3 technical replicates each. Data represented as mean ± SEM. One-way analysis of variance with Tukey’s post test. *p < 0.05. ns = not significant.