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. Author manuscript; available in PMC: 2024 Jan 25.
Published in final edited form as: Cell Rep. 2023 Dec 4;42(12):113535. doi: 10.1016/j.celrep.2023.113535

Figure 1. Kinase screen identifies GCK kinases as negative regulators of PI3Kα activity.

Figure 1.

(A) Experimental schema.

(B) Dendrogram of the human protein kinome that highlights the kinases selected for our screen.

(C) Thin-layer chromatography (TLC)-autoradiography of [32P]PIP3 produced by recombinant PI3Kα complexes (p110α/p85α, 500 nM) after incubation with the indicated recombinant protein kinases at 10 (L [low]) or 100 nM (H [high]) concentrations or with enzyme storage buffer (–).

(D) PIP2 kinase assays with PI3Kα after incubation with subset of the GCK family, performed as described in (C).

(E) PIP2 kinase assays with the class I PI3K isoforms (PI3Kα, PI3Kβ, PI3Kγ, and PI3Kδ, 500 nM) after incubation with MST1.

(D) and (E) are representative of two independent replicates.