KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Phospho-Akt (Ser473) Rabbit mAb | Cell Signaling Technology | Cat#3787; RRID:AB_331170 |
| Phospho-Akt (Ser473) Rabbit mAb | Cell Signaling Technology | Cat#4060; RRID:AB_2315049 |
| Phospho-Akt (Thr308) Rabbit mAb | Cell Signaling Technology | Cat#4056; RRID:AB_331163 |
| Phospho-Akt2 (Ser474) Rabbit mAb | Cell Signaling Technology | Cat#8599; RRID:AB_2630347 |
| Akt (pan) (40D4) Mouse mAb | Cell Signaling Technology | Cat#2920; RRID:AB_1147620 |
| AKT1 (C73H10) [#2967] | Cell Signaling Technology | Cat#2938; RRID:AB_915788 |
| Akt2 (D6G4) Rabbit mAb | Cell Signaling Technology | Cat#3063; RRID:AB_2225186 |
| Phospho-CREB (Ser133) Rabbit mAb | Cell Signaling Technology | Cat#9198; RRID:AB_2561044 |
| Phospho-LATS1 (Thr1079) Rabbit mAb | Cell Signaling Technology | Cat#8654; RRID:AB_10971635 |
| Phospho-MOB1 (Thr35) Rabbit mAb | Cell Signaling Technology | Cat#8699; RRID:AB_11139998 |
| Phospho-YAP (Ser127) Rabbit mAb | Cell Signaling Technology | Cat#13008; RRID:AB_2650553 |
| Phospho-PKA Substrate (RRXS*/T*) Rabbit mAb | Cell Signaling Technology | Cat#9624; RRID:AB_331817 |
| PI3 Kinase p110α (C73F8) Rabbit mAb | Cell Signaling Technology | Cat#4249; RRID:AB_2165248 |
| CREB (D76D11) Rabbit mAb | Cell Signaling Technology | Cat#4820; RRID:AB_1903940 |
| MOB1 (E1N9D) Rabbit mAb | Cell Signaling Technology | Cat#13730; RRID:AB_2783010 |
| LATS1 Antibody Rabbit Ab | Cell Signaling Technology | Cat#9153; RRID:AB_2296754 |
| MST1 (D8B9Q) Rabbit mAb | Cell Signaling Technology | Cat#14946; RRID:AB_2798654 |
| GAPDH (D16H11) Rabbit mAb | Cell Signaling Technology | Cat#5174; RRID:AB_10622025 |
| Recombinant Anti-STK3/MST-2 antibody [EP1466Y] | Abcam | Cat#ab52641; RRID:AB_882734 |
| p110 (pT1061) polyclonal antibody | This paper (from CST) | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Alpelisib (BYL-719) | MEDCHEM EXPRESS | Cat#HY-15244 |
| Neratinib (HKI-272) | SelleckChem | Cat#S2150 |
| Go6976 | EMD Millipore | Cat#365250 |
| DMX-5804 (HY-111754) | MEDCHEM EXPRESS | Cat#HY-111754 |
| XMU-MP-1 | SelleckChem | Cat#S8334 |
| H 89 dihydrochloride | Tocris Bioscience | Cat#2910 |
| AT13148 | SelleckChem | Cat#S7563 |
| Propranolol hydrochloride | Sigma Aldrich | Cat#P0884 |
| MST1 | SignalChem | S25–10G |
| HGK | SignalChem | M26–11G |
| MST2 | SignalChem | S24–10G |
| MST3 | SignalChem | S42–11G |
| TAOK1 | SignalChem | T24–11G |
| GLK | SignalChem | M25–11G |
| AKT1 | SignalChem | A16–10G |
| AMPK1 | SignalChem | P47–10H |
| ERK2 | SignalChem | M28–10G |
| PDK1 | SignalChem | P14–10H |
| CK1A | SignalChem | C64–10G |
| GSK3A | SignalChem | G08–10G |
| IKKB | SignalChem | I03–10BG |
| IRAK4 | SignalChem | I12–10G |
| ASK1 | SignalChem | M13–11G |
| GCN2 | SignalChem | E12–11G |
| EEF2K | SignalChem | E01–10G |
| JAK1 | SignalChem | J01–11G |
| CHK1 | SignalChem | C47–10G |
| CHK2 | SignalChem | C48–10G |
| DAPK1 | SignalChem | D01–11G |
| PKACA | SignalChem | P51–10G |
| PKCA | SignalChem | P61–18G |
| PAK1 | SignalChem | P02B-10BG |
| AurB | SignalChem | A31–10G |
| CDK1 | SignalChem | C22–10G |
| DYRK1A | SignalChem | D09–10G |
| SRC | SignalChem | S19–18G |
| ABL1 | SignalChem | A03–18H |
| LATS2 | Abnova | P5574 |
| OXSR1 | Abnova | P5614 |
| CK2 | NEB | P6010L |
| TGFBR1 | Proqinase | 0397-0000-1 |
| WEE1 | BPS Biosciences | 40412 |
| PIK3CG was purchased from Thermo Fisher | SignalChem | P27–10CG |
| Alpelisib (BYL-719) | MEDCHEM EXPRESS | Cat#HY-15244 |
| Critical commercial assays | ||
| Transcreener ADP fluorescence intensity assay | Bellbrook Labs | 3013-A |
| Deposited data | ||
| Crystal structure | Protein DataBank | entry D_1292124630 (8a.m.0) |
| Experimental models: Cell lines | ||
| MDA-MB-231 | ATCC | HTB-26 |
| Huh7 | Gift from Lab of Robert Schwartz | N/A |
| HEK293A | ATCC | CRL-1573 |
| MCF10A | ATCC | CRL-10317 |
| Primary mouse hepatocytes | isolated from mouse liver | N/A |
| Experimental models: Organisms/strains | ||
| Stk3f/f,Stk4f/f mice | Jackson laboratories | Stock No: 017635 |
| Liver specific MST1/2 double knockout mice (Stk3f/f,Stk4f/f,Alb-Cre−/+) | This paper | N/A |
| Albumin-Cre mice | Jackson laboratories | Stock No: 003574 |
| Homozygous null mice for the Adrb1 and Adrb2 genes (Adrb1−/−;Adrb2−/−) | Jackson laboratories | Stock No: 003810 |
| Pnpla2f/f mice | Jackson laboratories | Stock No: 024278 |
| Pik3caT1061A mice This paper | N/A | |
| Adipoq-Cre mice | Gift from Lab of James Lo | Stock No: 028020 |
| Oligonucleotides | ||
| PIK3CAV143I to WT isoleucine Forward: GACTTCCGAAGAAATATTCTGAACGTTTGTAAA | This paper | N/A |
| PIK3CAV143Ito WT isoleucine Reverse: TTTACAAACGTTCAGAATATTTCTTCGGAAGTC. | This paper | N/A |
| PIK3CAT1061A mutagenesis primer FW: 5′-caaaaatggattggatcttccacgcaattaaacagcatgcattgaac-3′ | This paper | N/A |
| PIK3CAT1061A mutagenesis primer RV: 5′-gttcaatgcatgctgtttaattgcgtggaagatccaatccatttttg-3 | This paper | N/A |
| PIK3CAT1061E mutagenesis primer FW: 5′-cctcagttcaatgcatgctgtttaatctcgtggaagatccaatccatttttgttg-3 | This paper | N/A |
| PIK3CAT1061E mutagenesis primer Rev: 5′-caacaaaaatggattggatcttccacgagattaaacagcatgcattgaactgagg-3 | This paper | N/A |
| PIK3CAW1057A mutagenesis primer FW: 5′-ctgtttaattgtgtggaagatcgcatccatttttgttgtccagcca-3′ | This paper | N/A |
| PIK3CAW1057A mutagenesis primer RV: 5′-tggctggacaacaaaaatggatgcgatcttccacacaattaaacag-3 | This paper | N/A |
| Recombinant DNA | ||
| pBabe puro HA-PIK3CA | Addgene | plasmid #12522 |
| Software and algorithms | ||
| Prism 7 | GraphPad La Jolla, CA | N/A |
| BioRender | https://biorender.com/ | N/A |
| Geneious | http://www.geneious.com/ | RRID:SCR_010519 |
| Chemdraw | http://www.perkinelmer.co.uk/category/chemdraw | RRID:SCR_016768 |
| Pymol | http://www.pymol.org/ | RRID:SCR_000305 |