TABLE 1.
Distribution of Arg-X enzyme activity in culture supernatants of P. gingivalis W50 and W50D7 (prR2 mutant)
| Fraction | Extracellular Arg-X enzyme activity (%)
|
|
|---|---|---|
| W50 | W50D7 (prR2 mutant) | |
| Culture supernatant | 100 | 100a |
| 85% Ammonium sulfate | ||
| Supernatant | 0 | 0 |
| Pellet | 100 | 100 |
| Solubilized with 0.0055% detergent | ||
| S-fraction (soluble) | 40 | 15 |
| ↓ | ||
| RI | 15 | 6.8 |
| RIA | 25 | 8.2 |
| Solubilized with 0.05% detergent | ||
| P-fraction (vesicle or membrane bound) | 60 | 85 |
| ↓ | ||
| RI | 10 | 51 |
| RIB | 50 | 34 |
a
Total extracellular Arg-X activity of P. gingivalis W50D7 (prR2 mutant) is approximately 50% of that of the parent strain W50.