ADCs with an average DAR of 4.
| ADC | Linker | DAR | DAR4 peak HIC RT/min | Agg/% |
|---|---|---|---|---|
| Ab | — | 0 | 3.37 | 1.6 |
| ADC1 | Ala–Ala | 3.8 | 4.90 | 1.0 |
| ADC2 | Ala–Arg | 3.6 | 4.52 | 1.1 |
| ADC3 | Ala–Gln | 3.7 | 4.83 | 1.8 |
| ADC4 | Ala–Glu | 3.3 | 4.65 | 1.3 |
| ADC5 | Ala–Gly | 3.7 | 4.77 | 2.1 |
| ADC6 | Ala–His | 3.5 | 4.90 | 1.7 |
| ADC7 | Ala–Lys | 2.4 | 4.18 | 1.9 |
| ADC8 | Ala–Ser | 4.2 | 4.82 | 1.4 |
| ADC9 | Gly–Ala | 3.8 | 4.80 | 1.7 |
| ADC10 | Gly–Gln | 3.7 | 4.71 | 1.3 |
| ADC11 | Gly–Glu | 3.2 | 4.57 | 1.9 |
| ADC12 | Gly–Gly | 3.6 | 4.74 | 1.9 |
| ADC13 | Gly–Ser | 3.7 | 4.69 | 1.3 |
| ADC14 | Phe–Lys | 3.7 | 4.68 | 3.1 |
| ADC15 | Ser–Ala | 3.8 | 4.87 | 1.8 |
| ADC16 | Ser–Asn | 3.5 | 4.72 | 1.5 |
| ADC17 | Ser–Gln | 3.6 | 4.79 | 1.1 |
| ADC18 | Ser–Glu | 3.5 | 4.63 | 1.7 |
| ADC19 | Ser–Gly | 3.7 | 4.77 | 1.8 |
| ADC20 | Val–Ala | 3.8 | 5.10 | 1.3 |
| ADC21 | Val–Arg | 3.4 | 4.73 | 1.7 |
| ADC22 | Val–Cit | 3.6 | 4.98 | 1.2 |
| ADC23 | Val–Asn | 3.5 | 4.90 | 1.1 |
| ADC24 | Val–Gln | 4.1 | 5.02 | 1.3 |
| ADC25 | Val–Glu | 3.4 | 4.79 | 2.0 |
| ADC26 | Val–Gly | 3.6 | 4.99 | 1.3 |
| ADC27 | Val–Ser | 3.7 | 4.98 | 1.5 |