. 2023 Nov 27;15(1):355–365. doi: 10.1039/d3md00473b

ADCs with a DAR of 10.

ADC	Dipeptide	DAR	DAR10 peak HIC RT/min	Agg/%	ADC yield/%
Ab	—	0.0	3.37	1.6	—
ADC28	Ala–Ala	10	6.52	2.8	55.3
ADC29	Ala–Arg	9.9	5.45	20.5	80.8
ADC30	Ala–Gln	9.9	6.38	3.1	9.9
ADC31	Ala–Glu	NA^a	NA^a	NA^a	2.5
ADC32	Ala–Gly	10	6.49	7.9	60.9
ADC33	Ala–His	9.6	6.33	13.5	70.8
ADC34	Ala–Ser	10	6.42	5.0	53.1
ADC35	Ala–Asn	10	6.45	6.3	29.9
ADC36	Ala–Val	10	6.92	13.6	49.7
ADC37	Ala–Phe	9.8	7.42	44.7	43.4
ADC38	Gly–Ala	10	6.29	7.7	51.0
ADC39	Gly–Gln	10	6.12	3.5	18.2
ADC40	Gly–Glu	NA^a	NA^a	NA^a	1.9
ADC41	Gly–Gly	10	6.26	8.2	54.1
ADC42	Gly–Ser	10	6.14	6.2	62.1
ADC43	Phe–Lys	9.8	5.72	50.9	70.5
ADC44	Ser–Ala	10	6.48	4.9	47.6
ADC45	Ser–Asn	9.6	6.19	12.2	38.3
ADC46	Ser–Gln	9.6	6.29	8.4	16.1
ADC47	Ser–Glu	NA^a	NA^a	NA^a	0.6
ADC48	Ser–Gly	10	6.33	6.1	43.2
ADC49	Val–Ala	10	6.74	12.9	45.7
ADC50	Val–Arg	9.6	5.77	36.4	68.9
ADC51	Val–Cit	9.9	6.54	21.5	54.4
ADC52	Val–Asn	9.8	6.46	13.9	40.8
ADC53	Val–Gln	10	6.66	5.8	10.2
ADC54	Val–Glu	NA^a	NA^a	NA^a	1.2
ADC55	Val–Gly	10	6.45	7.6	44.5
ADC56	Val–Ser	10	6.38	9.1	41.4

Not measured due to low sample recovery.