Fig. 6.

Reprogramming of the tumor microenvironment following treatment with TOFU-ACT. a UMAP of 15,000 CD45 + ve single cells from the TOFU-ACT and control groups which were resolved into 11 major clusters. The cell type and the percentage of cells are labeled across the respective population. b UMAP of T-cells and NK single cells from the TOFU-ACT and control groups which were resolved into 7 major clusters. The cell type and the percentage of cells are labeled across the respective population. c–d Analysis of gene expression of common T-cell exhaustion and cytotoxic markers in the three treatment groups across the different T-cell populations. The heatmap of gene expression is shown in c and the quantification of the same is shown in (d). Statistical analysis was performed using Kruskal–Wallis and Dunn’s test for multiple comparisons; p < 0.05 is *, p < 0.01 is **, p < 0.001 is ***, and p < 0.0001 is ****