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. 2024 Jan 25;24:55. doi: 10.1186/s12890-024-02869-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Vitamin D activates the Nrf2/HO-1 signaling pathway in mouse asthma. (a) qPCR was used to analyze Nrf2, HO-1 and NGF’s gene expression levels in lung tissue. (b) immunoblot analysis of the protein expression of Nrf2 and HO-1 in lung tissue. (c) gray-scale values count the relative protein expression of Nrf2 (nucleus) relative to Nrf2 (cytoplasm) and HO-1 relative to β-actin. (d) qPCR analysis of iNOS gene expression level in lung tissue. (e) immunoblot analysis of the protein expression of iNOS in lung tissue. (f) gray-scale values count the relative protein expression of iNOS relative to β-actin. (g) ELISA analysis of the NGF protein expression levels in BALF and serum. (* p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001)