FIG. 1.

Exoglycosidase treatment of IMTGP-1 and IMTGP-2. Intestinal brush border glycoproteins were subjected to treatment with no addition, neuraminidase from Arthrobacter ureafaciens (Neur.), β-galactosidase from E. coli (beta-Gal.), and sequentially with neuraminidase and β-galactosidase (Neur. + beta-Gal.) as described in Materials and Methods. K88ac adhesin binding activity (expressed as a percentage of the untreated control) was determined by BAOA, with the samples immobilized to polystyrene plates. The results shown are the means ± standard errors (n = 4) for untreated and exoglycosidase-treated intestinal brush border proteins.