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Purification of IMTGP-1 and IMTGP-2 receptors
| Purification step | Protein (mg) | Neutral sugars (μg/mg of protein) | Total units | Sp act (U/mg) | Receptor recovery (%) |
|---|---|---|---|---|---|
| Crude brush borders | 6,700 | 77.8 | 804 | 0.12 | |
| Phenol extract | 410 | 153.0 | 845 | 2.06 | 100 |
| IMTGP-1 | |||||
| ConA-Sepharose | 16.2 | 251.6 | 55 | 3.39 | 6.5 |
| Superose 12 | 0.7 | 640.0 | 3.1 | 4.30 | 0.4 |
| IMTGP-2 | |||||
| ConA-Sepharose | 87.4 | 438.0 | 332.1 | 3.80 | 39.3 |
| Jacalin agarose | 26.8 | 677.1 | 115.2 | 4.30 | 13.6 |
| Superose 12 | 9.4 | 943.0 | 39.5 | 4.20 | 4.7 |
