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. Author manuscript; available in PMC: 2024 Jan 25.

Published in final edited form as: Mol Cell Biochem. 2020 Jan 7;466(1-2):1–15. doi: 10.1007/s11010-019-03680-y

Fig. 9 — Plated MCs (a) and MGECs (b) on the chamber slides were treated with Ang-II for 48 h. GYY (GYY4137) was added 1 h prior to the Ang-II treatment. Following experiments, cells were immunostained against appropriate primary and fluorescence-tagged secondary antibodies against uPARAP/Endo180 (green) and Col IV (red), and nuclei counterstained with DAPI (blue). Co-localization of MMP-14 and Col IV are shown in the merged image. Scale bar, 20 μm, magnification 60 X. (c) and (d) summarized data of Endo 180 and Col IV expression in MCs and MGECs, respectively. Data are presented as mean ± SEM, n=9 independent experiments / group. *p <0.05 vs control, †p <0.05 vs Ang-II.

Fig. 9 — Plated MCs (a) and MGECs (b) on the chamber slides were treated with Ang-II for 48 h. GYY (GYY4137) was added 1 h prior to the Ang-II treatment. Following experiments, cells were immunostained against appropriate primary and fluorescence-tagged secondary antibodies against uPARAP/Endo180 (green) and Col IV (red), and nuclei counterstained with DAPI (blue). Co-localization of MMP-14 and Col IV are shown in the merged image. Scale bar, 20 μm, magnification 60 X. (c) and (d) summarized data of Endo 180 and Col IV expression in MCs and MGECs, respectively. Data are presented as mean ± SEM, n=9 independent experiments / group. *p <0.05 vs control, †p <0.05 vs Ang-II.