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Tcf expression by IEM101 under control of different promoters. Whole-cell lysates (corresponding to approximately 5 × 108 CFU of IEM101 or 5 × 107 CFU of BP18323) were fractionated by SDS-PAGE and probed with anti-Tcf antiserum. Samples shown are BP18323 (lane a), IEM101 (lane b), IEM101(pCT-tcf) (lane c), IEM101(pNIR-tcf) grown under aerobic conditions (lane d), and IEM101(pNIR-tcf) grown with low aeration (lane e).
