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. 1998 Apr;66(4):1648–1653. doi: 10.1128/iai.66.4.1648-1653.1998

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Copyright © 1998, American Society for Microbiology

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FIG. 3 — Tcf localization in IEM101. OMAP preparations from B. pertussis 18323 (from approximately 2 × 10⁸ CFU), outer membrane protein fractions from IEM101 (from approximately 10⁹ CFU), and trichloroacetic acid-precipitated culture supernatant (corresponding to 1 ml) were loaded on SDS-polyacrylamide gels and probed with anti-Tcf antiserum. Samples shown are B. pertussis 18323 OMAP (lane a), B. pertussis 18323 culture supernatant (lane b), IEM101 outer membrane fraction (lane c), IEM101(pNIR-tcf) outer membrane fraction (lane d), and IEM101(pNIR-tcf) culture supernatant (lane e).