Immune cell frequency in corneal transplantation. (A, C, E) Corneas were harvested at week 6 after corneal transplantation for flow cytometry. Representative images show CD4+ T cell, neutrophil, and macrophage populations in accepted or failed grafts, gated on CD45+ cells. (B, D, F) Frequency of CD4+ T cells, neutrophils, and macrophages. *P < 0.05, **P < 0.005 (n = 6/group; representative data from one of two repeats are shown).