Table 9.
Sequence-based PCR detection of different Phytophthora species infecting orchids with primer pairs.
| Pathogen identified | Orchid host | Locus | Forward primer name | Forward primer sequence | Reverse primer name | Reverse primer sequence | Reference |
|---|---|---|---|---|---|---|---|
| Phytophthora nicotianae | Dendrobium | ITS | ITS6 | 5′-GAAGGTGAAGTCGTAACAAGG-3′ | ITS4 | 5′-TCCTCCGCTTATTGATATGC-3′ | Zhang et al. (2006) |
| Dendrobium | ITS | ITS1 | 5′-TCCGTAGGTGAACCTGCGG-3′ | ITS2 | 5′-GCTGCGTTCTTCATCGATGC-3′ | Tao et al. (2011a) | |
| Dendrobium | Btub | tubuF2 | 5′-ACGGCTCGAGGATGACCATG-3′ | TubuR1 | 5′-CCTGGTACTGCTGGTACTCAG-3′ | Tao et al. (2011a) | |
| Vanilla | ITS | ITS1 | 5′-TCCGTAGGTGAACCTGCGG-3′ | ITS4 | 5′-TCCTCCGCTTATTGATATGC-3′ | Liu et al. (2008) | |
| Phytophthora multivesiculata | Cymbidium and Ansellia | COXI | FM84 | 5′-TTT AAT TTT TAG TGC TTT TGC-3′ | FM83 | 5′-CTCCAATAAAAAATAACCAAAAATG-3′ | Bose and Hammerbacher (2022) (Multiple gene test) |
| Cymbidium and Ansellia | Btub | tubuF1A | 5′-ACGGCTCGAGGATGACCATG-3′ | tubuR1 | 5′-CCTGGTACTGCTGGTACTCAG-3′ | ||
| Cymbidium and Ansellia | ITS | DC6 | 5′-GAGGGACTTTTGGGTAATCA-3′ | ITS4 | 5′-TCCTCCGCTTATTGATATGC-3′ | ||
| C. hybridum | ITS | ITS4 | 5′-TCCTCCGCTTATTGATATGC-3′ | ITS5 | 5′-GGAAGTAAAAGTCGTAACAAGG-3′ | Chern et al. (2011) | |
| Phytophthora palmivora | Cattleya | ITS1/ 28S | Phy1s | 5′-ACTTTCCACGTGAACCGTATCA-3′ | Phy2a | 5′-GCA CGA GCC ACT CAG GGA TG-3′ | Tsai et al. (2006) (Nested PCR) |
| ITS | ITS1 | 5′-CACGTGAACCGTATCAAAACT-3′ | ITS2 | 5′-CAA TCA TAC CAC CAC AGC TGA-3′ | |||
| Cattleya | ITS | ITS5 | 5′-GGAAGTAAAAGTCGTAACAAGG-3′ | ITS4 | 5′-TCCTCCGCTTATTGATATGC-3′ | Cating et al. (2010) | |
| Dinema | ITS | ITS5 | 5′-GAAGTAAAAGTCGTAACAAGG-3′ | ITS4 | 5′-CCTCCGCTTATTGATAGC-3′ | Suzuki et al. (2008) | |
| Rhynchostylis | ITS | ITS5 | 5′-GAAGTAAAAGTCGTAACAAGG-3′ | ITS4 | 5′-TCCTCCGCTTATTGATAGC-3′ | Wongwan et al. (2021) | |
| COXI | OomCoxILevup | 5′-TCAWCWMGATGGCTTTTTTCAAC-3′ | Fm85mod | 5′-RRHWACKTGACTDATRATACCAAA-3′ | |||
| Phytophthora capsici | Dendrobium | ITS | ITS1 | 5′-TCCGTAGGTGAACCTGCGG-3′ | ITS4 | 5′-TCCTCCGCTTATTGATATGC-3′ | Li et al. (2018) |
| CoxII | FM75F | 5′-CCTTGGCAATTAGGATTTCAAGAT-3′ | FM78R | 5′-CAAATTTCACTACATTGTCC-3′ | |||
| Phytophthora cactorum | Pleione | ITS | ITS1 | 5′-CACGTGAACCGTATCAAAACT-3′ | ITS2 | 5′-CAA TCA TAC CAC CAC AGC TGA-3′ | NCBI database |