Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Nov 30;52(2):977–997. doi: 10.1093/nar/gkad1125

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 4. — Lipid-conjugated P.O.s promote editing of C20, co-delivered with AAV vector expressing tracrRNA, in the CNS of Cas9-expressing reporter mice. (A) Schematic representation of the transgene constructs of Cas9/mTmG^+/+ double transgenic mouse model and the experimental process to test AAV co-delivery in vivo in the mouse CNS. Cleavage at sites flanking the mTmG tdTomato cassette activate EGFP expression. (B) Chemical modifications of the P.O.s used in this experiment. d(TT)-DHA, docosahexaenoic acid with a nuclease-cleavable d(TT)-PO-C7 linker; DHA, docosahexaenoic acid; d(TT)-TegChol, tetra-ethylene-glycol-linked cholesterol with a cleavable d(TT)-PO-C7 linker; TegChol, tetra-ethylene-glycol-linked cholesterol. (C) Immunohistochemistry (IHC) staining of mouse brain tissue sections using an anti-EGFP antibody. Adult Cas9/mTmG^+/+ mice were injected with scAAV9-tracrRNA and subsequently injected with C20 and different P.O.s as indicated on the upper left of each image. (D) ImageJ quantification of percentages of EGFP positive cells within 1 mm² area around the site of injection (n = 8 mice per group). Data represent mean ± SD; **** P < 0.0001 (one-way ANOVA).