Figure 1.
(A) Schematic of domains for the three different classes of PylRS enzymes (NTD, N-terminal domain; VL, variable linker; CTD, C-terminal domain). (B) Cloverleaf structures of Ca. M. alvus and M. mazei tRNAPyl (Ma and Mm, respectively) and depiction of the domains present in their cognate PylRS (MaPylRS and MmPylRS, respectively). Image representation is adapted from (47). MaPylRS is of the ΔpylSn class and MmPylRS is of the pylSn class. The numbers in the arrows represent the relative translation activity of the PylRS towards that tRNAPyl by our in vivo assays with Pyl as a substrate.