FIG. 3.

Total RNA extracted from mutant SBNH5 (lanes 2 to 7) and wild-type MGAS166s (lanes 8 to 13) was quantified, standardized, blotted, and probed with a PCR amplicon of sagA labeled with α-³²P. Lane 1, 0.16- to 1.77-kb RNA standard; lane 2, SBNH5 RNA harvested at mid-log phase; lanes 3 to 7, SBNH5 RNA at 2, 4, 6, 8, and 10 h post-mid-log phase, respectively; lane 8, MGAS166s RNA harvested at mid-log phase; lanes 9 to 13, MGAS166s RNA at 2, 4, 6, 8, and 10 h post-mid-log phase, respectively. The mutant strain is devoid of any transcripts from sagA, while the wild type contains sagA transcripts at all time points.