TABLE 1.

Bacterial strains and relevant properties

Strain	Relevant phenotypea	Reference	Comments
S. pyogenes
T18P	M18 Sts Tcs SLS+	32	Isolate associated with rheumatic fever
MGAS166	M1 Sts Tcs SLS+	26	Invasive clinical isolate
T18Ps	M18 Str Tcs SLS+	This paper	Spontaneous Strr derivative of T18P
MGAS166s	M1 Str Tcs SLS+	This paper	Spontaneous Str derivative of MGAS166
SB30-2	M18 Str Tcr SLS−	This paper	Nonhemolytic derivative of T18Ps
30-2rev	M18 Str Tcs SLS+	This paper	Hemolytic derivative of SB30-2
SBNH5	M1 Str Tcr SLS−	This paper	Nonhemolytic derivative of MGAS166s
NH5rev	M1 Str Tcs SLS+	This paper	Hemolytic derivative of SBNH5
ATCC 21547	SLS+ SLO+	NRb	Hemolytic control strain
ATCC 27762	SLS− SLO+	14	Nonhemolytic control strain
E. faecalis CG110	Sts Tcr	11	Tn916 donor strain
E. coli
RN6851	Tcr	NR	Contains pRN6680
DH5αMCR	mcrA φ80dlacΔZM15	Gibco BRL	Library efficiency competent cells
SL-1	Tcs Cmr	This paper	Contains pACYC184 with a 3.8-kb insert

^aSt^r, streptomycin resistant; St^s, streptomycin sensitive; Tc^s, tetracycline sensitive; Tc^r, tetracycline resistant; Cm^r, chloramphenicol resistant. 

^bNR, no reference.