Skip to main content
. 2024 Jan 25;19(1):e0297585. doi: 10.1371/journal.pone.0297585

Fig 1. Flow cytometric analysis of murine lung digests with lung inflation, comparing the number of cells after hot (1h, 37°C) or cold (20h, 4°C) digests.

Fig 1

(a) Representative gating strategy following debris exclusion (FSC/SSC), singlets (FSC-H/FSC-A) and dead cells exclusion (LIVE/DEAD fixable near-IR). CD45-CD31- cells are gated, followed by EpCAM+ gating. (b) Number of CD45-CD31-EpCAM+ AECs, CD31+ endothelial cells or CD45+ leukocytes. (c) Comparison of relative MFI (median) values for EpCAM, CD45 and CD31 between cold and hot digests within respective CD45-CD31-EpCAM+, CD45+ and CD31+ gates. Each data point represents a lung from a single mouse. Unpaired t-test, median ± min/max.