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. Author manuscript; available in PMC: 2024 Mar 1.
Published in final edited form as: Clin Immunol. 2022 Dec 22;248:109213. doi: 10.1016/j.clim.2022.109213

Figure 5. Differential renal lipid profile and increased esterification of phosphatidylethanolamine (PE) SN1 chain in nephritic mice.

Figure 5.

8-week-old (non-nephritic) and 20-week-old (nephritic) MRL/lpr female kidneys were analyzed by semi-targeted LS-MS for their lipid profile and content. Representative normal phase LC-MS/MS chromatogram for six major classes of phospholipids: phosphatidylcholine (PC), sphingomyelin

(SM),lysophophatidylcholine(PLC),phosphotidylethanolamine(PE),lysophosphotidylethanolamin e (LPE), phosphotidylglycerol (PG), and phosphotidylinostitol (PI) (A). Nephritic mice (20-week-old) had significantly higher concentrations of PE(P-18:0/20:4) (B), PE(P-18:1/20:4) (C) and PE(P-18:0/20:4) (D), the major storage depots for arachidonic acid. Nephritis was also associated with an increase in esterified C22 chain of PE (P-18:0/22:4), the preferred substrate for ferroptosis (E). (n = 6 each). Statistical significance was determined by 2-tailed Mann-Whitney test. Data are plotted as mean ± SEM *P < 0.05.